Signs to Tell You That You Need to Replace Your HPLC Column
You all know it can be frustrating when you run a precious sample on a column that you have used before for the same applications, only to get terrible performance leading to compromising your experiment. You may be running your final results after trying to optimize for months and then get results that are beyond recognition, you need to get an alert that you should act fast. Experts will conclude that it could be that your column is contaminated, you need to consider help here. Here are some of the diagnostic strategies that you can use when you are looking forward to determining how you can be able to choose the right HPLC column to ensure that you are safe all the time.
Check out if the cause may be due to contamination, you need to clean with the right reagents so that you do not result to contamination. Expert will tell you that after using all the time, the column needs to be stored in a miscible solvent for instance methanol. It is critical that you focus on using a solvent like methanol so that you can be able to enjoy the best services as this is one thing that has been featured mostly this time around and you need to take much care. Check out if the column has been equilibrated before you run it, for the best results, you need to have to equilibrate to get results that are perfect.
You need to know that having procedural ways of handling your clogged HPLC column is very important, you need to carry out some troubleshooting to resolve the issue. You need to carry out tests before you run your experiment through an external solubility or a dilution of the sample. In case you have tried and the insoluble parts are still there you know this can result to impacting the results in a negative manner as it matters so much in what you have been choosing.
The extracts can also be removed by the use of the strong solvent that happens to be compatible with what you have been offering, it will help you know if this is what is needed for you. Be sure that you also try to flush the materials using the reverse method, it can result to interfering the bed integrity, and this can, on the other hand, lead to tempering the performance, and it matters so much.
These issues will impact the general performance of the columns and overall evidence by running a calibration sample in the right manner, it will help you monitor the pressure level as well as consistency. If you find that the practices are not working through the troubleshooting it is time to replace your column, you will be able to boost better experiments in your laboratory.
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